TISSUE CULTURE (PART 3 : EXECUTION)

ACTIVITY 3

IMPLEMENTATION OF PLANT TISSUE CULTURE

I. INTRODUCTION
BackgroundTissue culture is one way of vegetative propagation of plants. Tissue culture propagation is a technique by isolating parts of plants such as leaves, buds, and growing these parts in an artificial medium aseptically rich nutrients and plant growth regulators in a closed opaque container so that the plants can reproduce themselves and generations to complete plants.

TISSUE CULTURE (PART 2: MAKING MEDIA)

ACTIVITY 2

 MAKING MEDIA

I. INTRODUCTION

A. Background 
In addition to tissue culture equipment, media is one major factor in the success of the culture. Plant tissue culture media should contain all the substances necessary to ensure the growth of explants grown.Tissue culture media have the characteristics of each.

TISSUE CULTURE (PART 1: Sterilization of Equipment)

ACTIVITY 1

Sterilization of equipment

I. INTRODUCTION
 

 A. Background 
Tissue culture is a method to isolate parts of plants such as protoplasm, cells, a group of cells, tissues or organs, as well as growing them in an aseptic, so that these parts can multiply and regenerate a whole plant back (Sany, 2007).